Animal Health Division

Mandate :

Development of preventive and control measures, packages of practices, alternative therapies and diagnostics for maintaining and improving health of goats

Patents Granted :

• An antibacterial herbal composition for animals (patent number: 327196 dated 11-12-2019).
• A herb based antibacterial preparation for veterinary use (patent number: 341364 dated 03-07-2020).
• A synergistic anti-bacterial herbal preparation for animals (patent number: 340760 dated 07-07-2020).
• AJAS green: Goat milk based natural herbal beauty soap (patent number: 364566 dated 13-04-2021).
• AJAS: Goat milk based natural beauty soap (patent number: 383967 dated 09-12-2021).
• Method for preparation for herbal anti stressor formulation for goat (patent number: 421306 dated 13-02-2023).
• A formulation having antibacterial herbal extract for animal use (patent number: 425919 dated 20-03-2023).
• AJAS antiseptic: Goat milk based natural herbal antiseptic soap (patent number: 433430 dated 31-05-2023).
• Method for development of herbal immunomodulator composition for goats (patent number: 446708 dated 23-08-2023) .

Products Commercialized

• Alquit   :     Ectoparasiticidal drug for animals
• HEALEX-FR   :    Skin antiseptic for animals
• Diarrionex-HS   :     Anti-diarrhoeal powder for animals
• Wormolex   :     Anthelminitic bolus for animals
• Megatex   :     Herbal acaricidal liquid/spray for animals
• IMU-4   :    Immunomodulatory bolus for animals
• AJAS   :     Goat milk based herbal fortified soap (three variants)

Herbal Products Developed

• Bruclear: It prevents shedding of Brucella melitensis, and treats brucellosis caused by Brucella melitensis in small ruminants.
• Sanjeevani: Herbal immunomodulator for weaned goat-kids .
• Easy Kidder: It ameliorates pregnancy stress in animals .
• Stressol - G: It ameliorates heat & cold stress in animals.

Diagnostic Tests Developed

BruLISA:   : IgG and IgM based indirect ELISA for detection of Brucella melitensis.

BruCheck:   : Dot-ELISA for quick and on-spot detection of Brucella melitensis.

BruTECT-SR^TM:   : OMP31 TaqMan® probe based RT-PCR for specific and quick detection of Brucella melitensis.

Visual LAMP Assay   : for quick detection of Brucella melitensis.

Latex Milk Agglutination Test:   : A field test for rapid diagnosis of Johne's disease (JD) .

Indigenous Dot-Elisa :   : A sensitive field based herd screening test for JD .

Nano-Immuno Test   : for early and rapid detection of live Mycobacterium avium subspecies paratuberculosis (MAP) bacilli in milk samples .

Novel Mce-Truncated Protein based ELISA  :  for diagnosis of JD .

Indirect Fluorescent Antibody Test:   : A milk screening test for JD .

Visual LAMP   : Assay for quick diagnosis of JD.

IgG based Indirect ELISA  for diagnosis of JD.

DIVA ELISA   : for differentiation of JD affected animals from vaccinated ones .

mRNA Multiplex TaqMan ® probe based RT-PCR   : for detection of live MAP bacilli in fecal samples .

EnteroLISA-ET^TM:  :  Microtiter plate based indirect ELISA that detects, and quantifies protective antibody titer in enterotoxaemia (ET) vaccinated goats.

ToxiScreen Pro^TM:   : Sandwich ELISA that can directly detect presence of epsilon toxin in suspected live and dead animals .

bfpA gene based RT-PCR   : for detection of enteropathogenic E. coli.

Latex Agglutination Test  :  for spot diagnosis of contagious caprine pleuropneumonia.

TaqMan® probe based RT-PCR   : for simultaneous detection of Group-A Rota and Bovine Corona viruses.

Conventional PCR   : targeting the major envelope glycoprotein gene for quick detection of contagious ecthyma .

mRNA based TaqMan® PCR   : for detection and differentiation of live thin walled and thick walled oocysts of Cryptosporidium species .

Peptide based Indirect ELISA   : for quick and early diagnosis of coenurosis in small ruminants and dogs.

Vaccines Developed

• Indigenous JD Vaccine (BioJD Gel ® and BioJD Oil®): It is killed vaccine, and was developed using " Indian Bison Type" genotype of Mycobacterium avium subspecies paratuberculosis strain " S5" of goat origin.
• Killed Vaccine against colibacillosis that provides passive lacteal protection in neonatal goat-kids.
• Currently, the work is going on the novel recombinant chimeric multi-epitope vaccine against Clostridium perfringens Type D causing enterotoxaemia in domestic ruminants.

Product/Method/Process/Procedure/Database Developed

• Plaque Assay for titration of PPR virus.
• Process to purify PPR and FMD viruses from the mixture of different viruses.
• Primary Goat Testes Cell Culture for quick isolation of Orf virus.
• DNA Extraction Protocol: for Cryptosporidium spp. oocysts present in fecal samples of goats .
• E. coli Phage-Cocktail for treatment of colibacillosis in goat-kids.
• Standard Operating Procedure for management of newborn goat-kids.
• Annual Health Calendar: Preventive Health Calendar for Goats.
• Para-Check Card: An eye mucosa color based targeted selective treatment chart against haemonchosis in goats .
• Faecal Consistency Score Chart for parasitic control in goats.
• Nucleotide Databases for ET caused by Clostridium perfringens Type D.

Recognitions/Awards

• DBT Fellowship for Research at Wisconsin University, USA.
• Japan International Cooperation Agency Fellowship.
• Visiting Scientist to USDA, USA.
• Post-Doctoral Research in Grenada, West Indies.
• National Research Development Corporation Award-2014 .
• Expert Member of Empowered Committee on Animal Health, GoI.
• Expert Member of Central Drugs Standard Control Organization, GoI .
• Board Member of International Goat Association representing Asia.
• Board Member of International Association of Para-TB.
• Fellowships (n=2) received from National Academy of Veterinary Sciences.
• Fellowships (n=5) received from Professional Societies ( ISSPGU, ISVM, ISVP & VIPM ).



Name of the Scientist	Designation	Discipline	E-mail address	EPBAX No.	View

1	Dr. K. Gururaj	Veterinary Microbiology	Principal Scientist (Head)	k.gururaj@icar.org.in	132	View
2	Dr. A. K. Mishra	Veterinary Microbiology	Senior.Scientist	anil.mishra79@icar.org.in	131	View
3	Dr.Nitika Sharma	Veterinary Medicine	Senior Scientist	nitika.sharma@icar.gov.in	130	View
4	Dr. Nisha Bisht	Veterinary Medicine	Scientist	nisha.bisht27@icar.org.in	128	View